Shifts in the host range of a promiscuous plasmid through parallel evolution of its replication initiation protein
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- Masahiro Sota
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
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- Hirokazu Yano
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
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- Julie M Hughes
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
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- Gary W Daughdrill
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
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- Zaid Abdo
- Departments of Mathematics and Statistics, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, Brink Hall , Moscow, ID , USA
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- Larry J Forney
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
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- Eva M Top
- Department of Biological Sciences, Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, 252 Life Sciences South , Moscow, ID , USA
説明
<jats:title>Abstract</jats:title> <jats:p>The ability of bacterial plasmids to adapt to novel hosts and thereby shift their host range is key to their long-term persistence in bacterial communities. Promiscuous plasmids of the incompatibility group P (IncP)-1 can colonize a wide range of hosts, but it is not known if and how they can contract, shift or further expand their host range. To understand the evolutionary mechanisms of host range shifts of IncP-1 plasmids, an IncP-1β mini-replicon was experimentally evolved in four hosts in which it was initially unstable. After 1000 generations in serial batch cultures under antibiotic selection for plasmid maintenance (kanamycin resistance), the stability of the mini-plasmid dramatically improved in all coevolved hosts. However, only plasmids evolved in Shewanella oneidensis showed improved stability in the ancestor, indicating that adaptive mutations had occurred in the plasmid itself. Complete genome sequence analysis of nine independently evolved plasmids showed seven unique plasmid genotypes that had various kinds of single mutations at one locus, namely, the N-terminal region of the replication initiation protein TrfA. Such parallel evolution indicates that this region was under strong selection. In five of the seven evolved plasmids, these trfA mutations resulted in a significantly higher plasmid copy number. Evolved plasmids were found to be stable in four other naive hosts, but could no longer replicate in Pseudomonas aeruginosa. This study shows that plasmids can specialize to a novel host through trade-offs between improved stability in the new host and the ability to replicate in a previously permissive host.</jats:p>
収録刊行物
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- The ISME Journal
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The ISME Journal 4 (12), 1568-1580, 2010-06-03
Oxford University Press (OUP)