Cellular Compartment Model for Exploring the Effect of the Lipidic Membrane on the Kinetics of Encapsulated Biochemical Reactions
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- Takeshi Sunami
- Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Kazufumi Hosoda
- Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Hiroaki Suzuki
- Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Tomoaki Matsuura
- Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Tetsuya Yomo
- Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan
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説明
One of the important characteristics of the cellular system is that interactions between the plasma membrane and water-soluble molecules in the cytoplasm are enhanced by the confinement of the molecules to the small volume of the intracellular space. Studying this effect in a model cell system, we measured the time evolution of an enzymatic hydrolysis reaction and a cell-free protein synthesis reaction taking place in giant liposomes having various size and phospholipid compositions by a flow cytometry. This single vesicle-based assay of a large number of liposomes enabled us to examine the volume dependence of enclosed reactions in detail, revealing that the presence of specific lipid affected the specific kinetic parameters of encapsulated reactions.
収録刊行物
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- Langmuir
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Langmuir 26 (11), 8544-8551, 2010-02-04
American Chemical Society (ACS)