<i>Fusarium oxysporum</i> mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics
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- Yashwant Kumar
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Limin Zhang
- Key Laboratory of Magnetic Resonance in Biological Systems National Centre for Magnetic Resonance in Wuhan Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan China
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- Priyabrata Panigrahi
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Bhushan B. Dholakia
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Veena Dewangan
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Sachin G. Chavan
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Shrikant M. Kunjir
- Central NMR Facility CSIR‐National Chemical Laboratory Pune India
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- Xiangyu Wu
- Key Laboratory of Magnetic Resonance in Biological Systems National Centre for Magnetic Resonance in Wuhan Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan China
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- Ning Li
- Key Laboratory of Magnetic Resonance in Biological Systems National Centre for Magnetic Resonance in Wuhan Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan China
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- Pattuparambil R. Rajmohanan
- Central NMR Facility CSIR‐National Chemical Laboratory Pune India
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- Narendra Y. Kadoo
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Ashok P. Giri
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
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- Huiru Tang
- Key Laboratory of Magnetic Resonance in Biological Systems National Centre for Magnetic Resonance in Wuhan Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan China
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- Vidya S. Gupta
- Division of Biochemical Sciences CSIR‐National Chemical Laboratory Pune India
抄録
<jats:title>Summary</jats:title><jats:p>Molecular changes elicited by plants in response to fungal attack and how this affects plant–pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and <jats:italic>Fusarium oxysporum</jats:italic> f. sp. <jats:italic>ciceri</jats:italic> (Foc), using quantitative label‐free proteomics and <jats:styled-content style="fixed-case">NMR</jats:styled-content>‐based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species (<jats:styled-content style="fixed-case">ROS</jats:styled-content>), lignification and phytoalexins. The levels of some of the pathogenesis‐related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations in the metabolic flux as outcome of several orchestrated molecular events are determinant of plant's role in chickpea–Foc interactions.</jats:p>
収録刊行物
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- Plant Biotechnology Journal
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Plant Biotechnology Journal 14 (7), 1589-1603, 2016-01-23
Wiley