Manipulation of Fatty Acid Composition in Animal Cells Grown in Culture

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  • Bernadine J. Wisnieski
    Department of Bacteriology and the Molecular Biology Institute, University of California, Los Angeles, Calif. 90024
  • Robert E. Williams
    Department of Bacteriology and the Molecular Biology Institute, University of California, Los Angeles, Calif. 90024
  • C. Fred Fox
    Department of Bacteriology and the Molecular Biology Institute, University of California, Los Angeles, Calif. 90024

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<jats:p> The fatty acid composition of animal cells cultured in serum-free medium can be manipulated when the synthesis of endogenous fatty acids is inhibited by a biotin analog and fatty acids are supplied in the medium as detergent esters of Tween. When mouse LM cells were grown in medium supplemented with Tween-19:0 (an ester of Tween and nonadecanoic acid), odd chain fatty acid content of cellular phospholipids and neutral lipids increased from 1% to 75%. Concurrently, the saturated fatty acid content increased from 27% to 85%. Similar alterations in fatty acid content have been observed when BHK <jats:sub>21</jats:sub> cells are subjected to the same enrichment regime. The ability to control the fatty acid composition of cultured animal cells is a prerequisite to investigations into the role of the membrane lipid physical state in processes unique to these cells. </jats:p>

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