The Pacific bluefin tuna (<i>Thunnus orientalis</i>) <i>dead end</i> gene is suitable as a specific molecular marker of type A spermatogonia
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- Ryosuke Yazawa
- Department of Marine Biosciences Tokyo University of Marine Science and Technology Tokyo Japan
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- Yutaka Takeuchi
- Research Center for Advanced Science and Technology Tokyo University of Marine Science and Technology Tateyama Japan
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- Tetsuro Morita
- Central Research Laboratory Nippon Suisan Kaisha Ltd. Tokyo Japan
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- Masashi Ishida
- Oita Marine Biological Technology Center Nippon Suisan Kaisha Ltd. Oita Japan
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- Goro Yoshizaki
- Department of Marine Biosciences Tokyo University of Marine Science and Technology Tokyo Japan
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<jats:title>Summary</jats:title><jats:sec><jats:label /><jats:p>We developed a spermatogonial transplantation technique to produce donor‐derived gametes in surrogate fish. Our ultimate aim is to establish surrogate broodstock that can produce bluefin tuna. We previously determined that only type A spermatogonia (ASG) could colonize recipient gonads in salmonids. Therefore, it is necessary to develop a precise molecular marker that can distinguish ASG in order to develop efficient spermatogonial transplantation methods. In this study, the Pacific bluefin tuna (<jats:italic>Thunnus orientalis</jats:italic>) <jats:italic>dead end</jats:italic> (<jats:italic>BFTdnd</jats:italic>) gene was identified as a specific marker for ASG. In situ hybridization and RT‐PCR analysis with various types of spermatogenic cell populations captured by laser microdissection revealed that localization of <jats:italic>BFTdnd</jats:italic> mRNA was restricted to ASG, and not detected in other differentiated spermatogenic cells. In order to determine if <jats:italic>BFTdnd</jats:italic> can be used as a molecular marker to identify germ cells with high transplantability, transplantation of dissociated testicular cells isolated from juvenile, immature, and mature Pacific bluefin tuna, which have different proportions of <jats:italic>dnd</jats:italic>‐positive ASG, were performed using chub mackerel as the surrogate recipient species. Colonization of transplanted donor germ cells was only successful with testicular cells from immature Pacific Bluefin tuna, which contained higher proportions of <jats:italic>dnd</jats:italic>‐positive ASG than juvenile and mature fish. Thus, <jats:italic>BFTdnd</jats:italic> is a useful tool for identifying highly transplantable ASG for spermatogonial transplantation. <jats:italic>Mol. Reprod. Dev. 80: 871–880, 2013. © 2013 Wiley Periodicals, Inc</jats:italic>.</jats:p></jats:sec>
収録刊行物
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- Molecular Reproduction and Development
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Molecular Reproduction and Development 80 (10), 871-880, 2013-08-26
Wiley
