Differential fates of biomolecules delivered to target cells via extracellular vesicles
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- Masamitsu Kanada
- Departments of aPediatrics,
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- Michael H. Bachmann
- Departments of aPediatrics,
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- Jonathan W. Hardy
- Departments of aPediatrics,
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- Daniel Omar Frimannson
- Microbiology and Immunology, and
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- Laura Bronsart
- Departments of aPediatrics,
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- Andrew Wang
- Departments of aPediatrics,
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- Matthew D. Sylvester
- Microbiology and Immunology, and
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- Tobi L. Schmidt
- Departments of aPediatrics,
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- Roger L. Kaspar
- TransDerm Inc., Santa Cruz, CA 95060
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- Manish J. Butte
- Departments of aPediatrics,
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- A. C. Matin
- Microbiology and Immunology, and
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- Christopher H. Contag
- Departments of aPediatrics,
Description
<jats:title>Significance</jats:title> <jats:p>Extracellular vesicle (EV)-mediated transfer of macromolecules may play a key role in cellular communication and may have utility in directed molecular therapies. In addition, the EV packaged biomolecules in serum may have potential for diagnosing cancer and determining its likelihood of metastasis. EVs are heterogeneous and there are many outstanding questions associated with biogenesis, uptake, and the fate of transferred molecules in recipient cells. In fact, the function, characterization, and even the nomenclature of EVs are being refined. Here we aimed to improve the functional characterization of EVs, and observed that only microvesicles (MVs), but not exosomes, can functionally transfer loaded reporter molecules to recipient cells, largely by delivering plasmid DNA. Our data show that exosomes and MVs are structurally and functionally distinct.</jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 112 (12), E1433-, 2015-02-23
Proceedings of the National Academy of Sciences
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Details 詳細情報について
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- CRID
- 1360292621032551680
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- ISSN
- 10916490
- 00278424
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- Data Source
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- Crossref