The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin in cis
-
- Anat Melamed
- Division of Infectious Diseases, Imperial College London, London, United Kingdom
-
- Hiroko Yaguchi
- Division of Infectious Diseases, Imperial College London, London, United Kingdom
-
- Michi Miura
- Division of Infectious Diseases, Imperial College London, London, United Kingdom
-
- Aviva Witkover
- Division of Infectious Diseases, Imperial College London, London, United Kingdom
-
- Tomas W Fitzgerald
- The European Bioinformatics Institute (EMBL-EBI), Cambridge, United Kingdom
-
- Ewan Birney
- The European Bioinformatics Institute (EMBL-EBI), Cambridge, United Kingdom
-
- Charles RM Bangham
- Division of Infectious Diseases, Imperial College London, London, United Kingdom
説明
<jats:p>Chromatin looping controls gene expression by regulating promoter-enhancer contacts, the spread of epigenetic modifications, and the segregation of the genome into transcriptionally active and inactive compartments. We studied the impact on the structure and expression of host chromatin by the human retrovirus HTLV-1. We show that HTLV-1 disrupts host chromatin structure by forming loops between the provirus and the host genome; certain loops depend on the critical chromatin architectural protein CTCF, which we recently discovered binds to the HTLV-1 provirus. We show that the provirus causes two distinct patterns of abnormal transcription of the host genome in cis: bidirectional transcription in the host genome immediately flanking the provirus, and clone-specific transcription in cis at non-contiguous loci up to >300 kb from the integration site. We conclude that HTLV-1 causes insertional mutagenesis up to the megabase range in the host genome in >104 persistently-maintained HTLV-1+ T-cell clones in vivo.</jats:p>
収録刊行物
-
- eLife
-
eLife 7 :e36245-, 2018-06-26
eLife Sciences Publications, Ltd