Targeted base editing in the plastid genome of Arabidopsis thaliana
Description
<jats:title>Abstract</jats:title><jats:p>Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells<jats:sup>1</jats:sup>. We applied this system to targeted base editing in the <jats:italic>Arabidopsis thaliana</jats:italic> plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T<jats:sub>1</jats:sub> generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.</jats:p>
Journal
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- Nature Plants
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Nature Plants 7 (7), 906-913, 2021-07-01
Springer Science and Business Media LLC
