An “OFF–ON–OFF” fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems

  • Shahi Imam Reja
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Yuichiro Hori
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Takuya Kamikawa
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Kohei Yamasaki
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Miyako Nishiura
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Steven D. Bull
    Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK
  • Kazuya Kikuchi
    Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan

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Description

<jats:p>An “OFF–ON–OFF” fluorescence probe for real-time imaging of the expression (fluorescence ‘OFF’) and degradation (fluorescence ‘ON’) of short lived PYP-tag proteins in cellular systems.</jats:p>

Journal

  • Chemical Science

    Chemical Science 13 (5), 1419-1427, 2022

    Royal Society of Chemistry (RSC)

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