Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

Hugo Pereira, Luísa Barreira, André Mozes, Cláudia Florindo, Cristina Polo, Catarina V Duarte, Luísa Custódio, João Varela

doi:10.1186/1754-6834-4-61

Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

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説明

<jats:title>Abstract</jats:title> <jats:p>We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-<jats:italic>s</jats:italic>-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.</jats:p>