Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis

DOI PDF 被引用文献4件
  • Linda Viol
    Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany 1
  • Shoji Hata
    Centre for Cell and Molecular Biology, German Cancer Research Centre-Centre for Cell and Molecular Biology Alliance, University of Heidelberg, Heidelberg, Germany 3
  • Ana Pastor-Peidro
    Centre for Cell and Molecular Biology, German Cancer Research Centre-Centre for Cell and Molecular Biology Alliance, University of Heidelberg, Heidelberg, Germany 3
  • Annett Neuner
    Centre for Cell and Molecular Biology, German Cancer Research Centre-Centre for Cell and Molecular Biology Alliance, University of Heidelberg, Heidelberg, Germany 3
  • Florian Murke
    Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany 4
  • Patrick Wuchter
    Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany 5
  • Anthony D. Ho
    Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany 5
  • Bernd Giebel
    Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany 4
  • Gislene Pereira
    Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany 1

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<jats:p>Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in a manner that relies upon Nek2 kinase activity to ensure low DA levels at mitotic centrosomes. Overexpression of active Nek2A, but not kinase-dead Nek2A, prematurely displaced DAs from the interphase centrosomes of immortalized retina pigment epithelial (RPE1) cells. This dramatic impact was also observed in mammary epithelial cells with constitutively high levels of Nek2. Conversely, Nek2 knockout led to incomplete dissociation of DAs and cilia in mitosis. As a consequence, we observed the presence of a cilia remnant that promoted the asymmetric inheritance of ciliary signaling components and supported cilium reassembly after cell division. Together, our data establish Nek2 as an important kinase that regulates DAs before mitosis.</jats:p>

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