Immunohistochemical Analysis Using Monoclonal Antibody PMab-269 Against Steller Sea Lion Podoplanin

  • Guanjie Li
    Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Hiroyuki Suzuki
    Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Junko Takei
    Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Masaki Saito
    Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Nohara Goto
    Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kazuyuki Uchida
    Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Japan.
  • Takayuki Nakagawa
    Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Japan.
  • Hiroyuki Harada
    Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
  • Tomohiro Tanaka
    Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Teizo Asano
    Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Mika K. Kaneko
    Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Yukinari Kato
    Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.

書誌事項

公開日
2022-02
資源種別
journal article
権利情報
  • https://journals.sagepub.com/page/policies/text-and-data-mining-license
DOI
  • 10.1089/mab.2021.0055
公開者
SAGE Publications

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説明

<jats:p>Monoclonal antibodies (mAbs) that specifically target podoplanin (PDPN), a marker for type I alveolar cells, are required for immunohistochemical analyses. Anti-PDPN mAbs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, Tasmanian devil, alpaca, tiger, whale, goat, horse, bear, sheep, and California sea lion PDPNs. However, no anti-Steller sea lion PDPN (stePDPN) antibody has been developed. Immunohistochemical analysis showed that an anti-California sea lion PDPN mAb (PMab-269) reacted with type I alveolar cells from the Steller sea lion lung, renal glomeruli and Bowman's capsules from kidney, and lymphatic endothelial cells from the colon, indicating that PMab-269 is useful for detecting stePDPN.</jats:p>

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