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- Eduardo Kenji Nunes Arashiro
- Universidade Federal Fluminense (UFF), Brazil
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- Pedro Henrique Nicolau Pinto
- Universidade Federal Fluminense (UFF), Brazil
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- Julia Timponi Moura Lima
- Universidade Federal Fluminense (UFF), Brazil
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- Marc Henry
- Universidade Federal de Minas Gerais (UFMG), Brazil
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- Fábio Otero Ascoli
- Universidade Federal Fluminense (UFF), Brazil
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- Joanna Maria Gonçalves Souza-Fabjan
- Universidade Federal Fluminense (UFF), Brazil
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- Jeferson Ferreira Da Fonseca
- Embrapa Caprinos e Ovinos, Brazil
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- Felipe Zandonadi Brandão
- Universidade Federal Fluminense (UFF), Brazil
抄録
<jats:p>ABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep.</jats:p>
収録刊行物
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- Ciência Rural
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Ciência Rural 52 (8), 2022
FapUNIFESP (SciELO)