Standardized Viral Hemagglutination and Hemagglutination-Inhibition Tests. II. Description and Statistical Evaluation

DOI Web Site 1 Citations
  • John C. Hierholzer
    Laboratory Division, National Communicable Disease Center, Atlanta, Georgia 30333
  • Morris T. Suggs
    Laboratory Division, National Communicable Disease Center, Atlanta, Georgia 30333
  • Elmer C. Hall
    Laboratory Division, National Communicable Disease Center, Atlanta, Georgia 30333

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Description

<jats:p>Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.</jats:p>

Journal

  • Applied Microbiology

    Applied Microbiology 18 (5), 824-833, 1969-11

    American Society for Microbiology

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