<jats:p> In this investigation, we examined the effects of listening to non-preferred (neutral) or self-selected motivational music while warming-up for the Wingate test at varied times of day. Participants were 10 male physical education students who were randomly assigned in a counterbalanced order to perform the Wingate test after a 10-minute warm-up with (a) self-selected motivational music (WUMM), (b) non-preferred music (WUNPM) or (c) no music (WUWM) at morning (0700) or afternoon (1700) times of day. We measured their peak powers (PP) and mean powers (MP) during the Wingate test, and we measured their ratings of perceived exertion (RPE) immediately after each of the warm-up and Wingate performances. PP and MP were higher in the afternoon, compared to the morning for all conditions. Both WUNPM and WUMM conditions were associated with enhanced PP in the morning (WUNPM: p < 0.001, d = 1.82; WUMM: p < 0.001, d = 2.59) and in the afternoon (WUNPM: p < 0.001, d = 1.24; WUMM: p < 0.01, d = 1.76) compared to WUWM, with greater enhancements after WUMM (0700: p < 0.05, d = 0.77; 1700: p < 0.05, d = 0.81) than after WUNPM. After the Wingate test, participants reported lower RPE scores for the WUMM condition, compared to either the WUWM condition (0700: p < 0.001, d = 0.20; 1700: p < 0.001, d = 0.84) or the WUNPM condition (0700: p < 0.01, d = 0.10; 1700: p < 0.05, d = 0.79). Thus, a warm-up with self-selected motivational music improved muscle power and decreased perceived exertion at both time points, with greater improvements at the morning hour (0700). </jats:p>