A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants
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- Mingming Chen
- Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Naoyoshi Kumakura
- Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science
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- Hironori Saito
- Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Ryan Muller
- Department of Molecular and Cell Biology, University of California, Berkeley
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- Madoka Nishimoto
- Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research
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- Mari Mito
- RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research
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- Pamela Gan
- Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science
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- Nicholas T Ingolia
- Department of Molecular and Cell Biology, University of California, Berkeley
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- Ken Shirasu
- Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science
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- Takuhiro Ito
- Laboratory for Translation Structural Biology, RIKEN Center for Biosystems Dynamics Research
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- Yuichi Shichino
- RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research
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- Shintaro Iwasaki
- Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo
抄録
<jats:p>Plants often generate secondary metabolites as defense mechanisms against parasites. Although some fungi may potentially overcome the barrier presented by antimicrobial compounds, only a limited number of examples and molecular mechanisms of resistance have been reported. Here, we found an <jats:italic>Aglaia</jats:italic> plant-parasitizing fungus that overcomes the toxicity of rocaglates, which are translation inhibitors synthesized by the plant, through an amino acid substitution in a eukaryotic translation initiation factor (eIF). <jats:italic>De novo</jats:italic> transcriptome assembly revealed that the fungus belongs to the <jats:italic>Ophiocordyceps</jats:italic> genus and that its eIF4A, a molecular target of rocaglates, harbors an amino acid substitution critical for rocaglate binding. Ribosome profiling harnessing a cucumber-infecting fungus, <jats:italic>Colletotrichum orbiculare</jats:italic>, demonstrated that the translational inhibitory effects of rocaglates were largely attenuated by the mutation found in the <jats:italic>Aglaia</jats:italic> parasite. The engineered <jats:italic>C. orbiculare</jats:italic> showed a survival advantage on cucumber plants with rocaglates. Our study exemplifies a plant–fungus tug-of-war centered on secondary metabolites produced by host plants.</jats:p>
収録刊行物
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- eLife
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eLife 12 e81302-, 2023-02-28
eLife Sciences Publications, Ltd