Impact on quinolone resistance of plasmid-mediated quinolone resistance gene and mutations in quinolone resistance-determining regions in extended spectrum beta lactamase-producing <i>Klebsiella pneumoniae</i> isolated from urinary tract infection patients
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- Reo Onishi
- Department of Public Health, Kobe University Graduate School of Health Sciences , 7-10-2 Tomogaoka , Suma-ku, Kobe 654-0142, Japan
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- Katsumi Shigemura
- Department of Public Health, Kobe University Graduate School of Health Sciences , 7-10-2 Tomogaoka , Suma-ku, Kobe 654-0142, Japan
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- Kayo Osawa
- Department of Medical Technology, Kobe Tokiwa University , 2-6-2 Otani-cho , Nagata-ku, Kobe 653-0838, Japan
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- Young-Min Yang
- Division of Urology, Kobe University Graduate School of Medicine , 7-5-2 Kusunoki-cho , Chuo-ku, Kobe 650-0017, Japan
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- Koki Maeda
- Division of Urology, Kobe University Graduate School of Medicine , 7-5-2 Kusunoki-cho , Chuo-ku, Kobe 650-0017, Japan
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- Hiroshi Tanimoto
- Department of Public Health, Kobe University Graduate School of Health Sciences , 7-10-2 Tomogaoka , Suma-ku, Kobe 654-0142, Japan
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- Mitsuki Kado
- Department of Public Health, Kobe University Graduate School of Health Sciences , 7-10-2 Tomogaoka , Suma-ku, Kobe 654-0142, Japan
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- Shiuh-Bin Fang
- Department of Pediatrics, Division of Pediatric Gastroenterology and Hepatology, Shuang Ho Hospital, Taipei Medical University , 291 Jhong Jheng Road , Jhong Ho District, New Taipei City 23561, Taiwan
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- Shian-Ying Sung
- International Ph.D. Program for Translational Science, College of Medical Science and Technology, Taipei Medical University , Taipei 11031, Taiwan
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- Takayuki Miyara
- Department of Infection Control and Prevention, Kobe University Hospital , 7-5-2 Kusunoki-cho , Chuo-ku, Kobe 650-0017, Japan
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- Masato Fujisawa
- Division of Urology, Kobe University Graduate School of Medicine , 7-5-2 Kusunoki-cho , Chuo-ku, Kobe 650-0017, Japan
説明
<jats:title>ABSTRACT</jats:title> <jats:p>Klebsiella pneumoniae is a typical pathogen in urinary tract infections (UTI), and the emergence of extended spectrum beta-lactamase (ESBL)-producing strains has been frequently reported, accompanied by higher quinolone resistance rates. There are two major mechanisms of quinolone resistance, mutations in quinolone resistance-determining regions (QRDR) and the presence of the plasmid-mediated quinolone resistance (PMQR) genes. This study aimed to investigate quinolone resistance among 105 ESBL-producing K. pneumoniae specimens isolated from UTI patients in Indonesia. These were characterized for antimicrobial resistance to nalidixic acid, ciprofloxacin, and levofloxacin, QRDR mutations in gyrA and parC and the presence of PMQR genes. We found that 84.8% of the collected isolates were resistant to at least one of the quinolones. QRDR mutation in gyrA was observed in 49.5% of these strains and parC mutations in 61.0%. PMQR genes were identified in 84.8% of strains. The QRDR mutations clearly had a greater effect on resistance than the PMQR genes. In conclusion, we found high quinolone resistance rates in Indonesian ESBL-producing K. pneumoniae, in which QRDR mutation played a major role.</jats:p>
収録刊行物
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- Pathogens and Disease
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Pathogens and Disease 80 (1), 2022
Oxford University Press (OUP)
