Disclosing quantitative <scp>RT‐PCR</scp> raw data during manuscript submission: a call for action

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  • Andreas Untergasser
    Zentrum für Molekulare Biologie der Universität Heidelberg Germany
  • Jan Hellemans
    CellCarta Zwijnaarde Belgium
  • Michael W. Pfaffl
    Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan Technical University of Munich Freising Germany
  • Jan M. Ruijter
    Department of Medical Biology, Amsterdam UMC, location AMC University of Amsterdam The Netherlands
  • Maurice J. B. van den Hoff
    Department of Medical Biology, Amsterdam UMC, location AMC University of Amsterdam The Netherlands
  • Mihnea P. Dragomir
    Institute of Pathology, Berlin Institute of Health Charité ‐ Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt‐Universität zu Berlin Germany
  • Douglas Adamoski
    Brazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas Brazil
  • Sandra Martha Gomes Dias
    Brazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas Brazil
  • Rui Manuel Reis
    Molecular Oncology Research Center Barretos Cancer Hospital Brazil
  • Manuela Ferracin
    Department of Medical and Surgical Sciences (DIMEC) University of Bologna Italy
  • Emmanuel Dias‐Neto
    Laboratory of Medical Genomics Centro Internacional de Pesquisa, AC Camargo Cancer Center São Paulo Brazil
  • Ian Marsh
    New South Wales Department of Primary Industries Elizabeth Macarthur Agricultural Institute Narellan Australia
  • Mikael Kubista
    TATAA Biocenter AB Göteborg Sweden
  • Muller Fabbri
    Center for Cancer and Immunology Research Children's National Hospital Washington DC USA
  • Ajay Goel
    Department of Molecular Diagnostics and Experimental Therapeutics Beckman Research Institute of City of Hope Monrovia CA USA
  • Ondřej Slabý
    Department of Biology, Faculty of Medicine, Central European Institute of Technology Masaryk University Brno Czech Republic
  • Erik Knutsen
    Department of Medical Biology, Faculty of Health Sciences UiT‐The Arctic University of Norway Tromsø Norway
  • Baoqing Chen
    Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐sen University Cancer Center Guangzhou China
  • Massimo Negrini
    Laboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine University of Ferrara Italy
  • Koshi Mimori
    Department of Surgery Kyushu University Beppu Hospital Japan
  • Martin Pichler
    Division of Oncology, Department of Internal Medicine Medical University of Graz Austria
  • Maria Papatriantafyllou
    Editorial Manager Molecular Oncology Cambridge UK
  • Simone Anfossi
    Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX USA
  • Thomas D. Schmittgen
    Department of Pharmaceutics University of Florida College of Pharmacy Gainesville FL USA
  • Jim Huggett
    National Measurement Laboratory LGC Teddington UK
  • Stephen Bustin
    Medical Technology Research Centre Anglia Ruskin University Chelmsford Essex UK
  • Jo Vandesompele
    CellCarta Zwijnaarde Belgium
  • George A. Calin
    Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX USA

説明

<jats:p>Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT‐qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT‐qPCR raw data. The Real‐time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.</jats:p>

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