Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection
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- Camilla D. de Mattos
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Dominick R. Faith
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Artem A. Nemudryi
- Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717
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- Amelia K. Schmidt
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- DeAnna C. Bublitz
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Lauren Hammond
- Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620
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- Margie A. Kinnersley
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Caleb M. Schwartzkopf
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Autumn J. Robinson
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Alex Joyce
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Lia A. Michaels
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Robert S. Brzozowski
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Alison Coluccio
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Denghui David Xing
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Jumpei Uchiyama
- Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan
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- Laura K. Jennings
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
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- Prahathees Eswara
- Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620
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- Blake Wiedenheft
- Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717
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- Patrick R. Secor
- Division of Biological Sciences, University of Montana, Missoula, MT 59812
抄録
<jats:p> Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, the danger signals released by dying bacteria and the mechanisms bacteria use for threat assessment remain largely unexplored. Here <jats:italic>,</jats:italic> we show that lysis of <jats:italic>Pseudomonas aeruginosa</jats:italic> cells releases polyamines that are subsequently taken up by surviving cells via a mechanism that relies on Gac/Rsm signaling. While intracellular polyamines spike in surviving cells, the duration of this spike varies according to the infection status of the cell. In bacteriophage-infected cells, intracellular polyamines are maintained at high levels, which inhibits replication of the bacteriophage genome. Many bacteriophages package linear DNA genomes and linear DNA is sufficient to trigger intracellular polyamine accumulation, suggesting that linear DNA is sensed as a second danger signal. Collectively, these results demonstrate how polyamines released by dying cells together with linear DNA allow <jats:italic>P. aeruginosa</jats:italic> to make threat assessments of cellular injury. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 120 (9), 2023-02-21
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1360298757449032704
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- ISSN
- 10916490
- 00278424
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- データソース種別
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- Crossref
- KAKEN