Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection

DOI Web Site 参考文献61件 オープンアクセス
  • Camilla D. de Mattos
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Dominick R. Faith
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Artem A. Nemudryi
    Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717
  • Amelia K. Schmidt
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • DeAnna C. Bublitz
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Lauren Hammond
    Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620
  • Margie A. Kinnersley
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Caleb M. Schwartzkopf
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Autumn J. Robinson
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Alex Joyce
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Lia A. Michaels
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Robert S. Brzozowski
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Alison Coluccio
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Denghui David Xing
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Jumpei Uchiyama
    Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan
  • Laura K. Jennings
    Division of Biological Sciences, University of Montana, Missoula, MT 59812
  • Prahathees Eswara
    Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620
  • Blake Wiedenheft
    Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717
  • Patrick R. Secor
    Division of Biological Sciences, University of Montana, Missoula, MT 59812

抄録

<jats:p> Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, the danger signals released by dying bacteria and the mechanisms bacteria use for threat assessment remain largely unexplored. Here <jats:italic>,</jats:italic> we show that lysis of <jats:italic>Pseudomonas aeruginosa</jats:italic> cells releases polyamines that are subsequently taken up by surviving cells via a mechanism that relies on Gac/Rsm signaling. While intracellular polyamines spike in surviving cells, the duration of this spike varies according to the infection status of the cell. In bacteriophage-infected cells, intracellular polyamines are maintained at high levels, which inhibits replication of the bacteriophage genome. Many bacteriophages package linear DNA genomes and linear DNA is sufficient to trigger intracellular polyamine accumulation, suggesting that linear DNA is sensed as a second danger signal. Collectively, these results demonstrate how polyamines released by dying cells together with linear DNA allow <jats:italic>P. aeruginosa</jats:italic> to make threat assessments of cellular injury. </jats:p>

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