Functional interrelationships between carbohydrate and lipid storage, and mitochondrial activity during sporulation in <i>Saccharomyces cerevisiae</i>

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  • Yanjie Liu
    Department of Cell Biology University of Texas Southwestern Medical Center Dallas TX USA
  • N. Ezgi Wood
    Department of Cell Biology University of Texas Southwestern Medical Center Dallas TX USA
  • Ashley J. Marchand
    Department of Cell Biology University of Texas Southwestern Medical Center Dallas TX USA
  • Orlando Arguello‐Miranda
    Department of Cell Biology University of Texas Southwestern Medical Center Dallas TX USA
  • Andreas Doncic
    Department of Cell Biology University of Texas Southwestern Medical Center Dallas TX USA

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<jats:title>Abstract</jats:title><jats:p>In <jats:italic>Saccharomyces cerevisiae</jats:italic> under conditions of nutrient stress, meiosis precedes the formation of spores. Although the molecular mechanisms that regulate meiosis, such as meiotic recombination and nuclear divisions, have been extensively studied, the metabolic factors that determine the efficiency of sporulation are less understood. Here, we have directly assessed the relationship between metabolic stores and sporulation in <jats:italic>S. cerevisiae</jats:italic> by genetically disrupting the synthetic pathways for the carbohydrate stores, glycogen (<jats:italic>gsy1/2Δ</jats:italic> cells), trehalose (<jats:italic>tps1Δ</jats:italic> cells), or both (<jats:italic>gsy1/2Δ</jats:italic> and <jats:italic>tps1Δ</jats:italic> cells). We show that storage carbohydrate‐deficient strains are highly inefficient in sporulation. Although glycogen and trehalose stores can partially compensate for each other, they have differential effects on sporulation rate and spore number. Interestingly, deletion of the G<jats:sub>1</jats:sub> cyclin, <jats:italic>CLN3</jats:italic>, which resulted in an increase in cell size, mitochondria and lipid stores, partially rescued meiosis progression and spore ascus formation but not spore number in storage carbohydrate‐deficient strains. Sporulation efficiency in the carbohydrate‐deficient strain exhibited a greater dependency on mitochondrial activity and lipid stores than wild‐type yeast. Taken together, our results provide new insights into the complex crosstalk between metabolic factors that support gametogenesis.</jats:p>

収録刊行物

  • Yeast

    Yeast 37 (3), 269-279, 2020-01-27

    Wiley

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