Constructing a yeast to express the largest cellulosome complex on the cell surface
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- Marimuthu Anandharaj
- Biodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan;
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- Yu-Ju Lin
- Biodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan;
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- Rizwana Parveen Rani
- Biodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan;
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- Eswar Kumar Nadendla
- Institute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan;
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- Meng-Chiao Ho
- Institute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan;
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- Chieh-Chen Huang
- Department of Life Sciences, National Chung Hsing University, 40227 Taichung, Taiwan;
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- Jan-Fang Cheng
- Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, CA 94598;
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- Jui-Jen Chang
- Department of Medical Research, China Medical University Hospital, China Medical University, 402 Taichung, Taiwan;
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- Wen-Hsiung Li
- Biodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan;
説明
<jats:title>Significance</jats:title> <jats:p> Sustainable utilization of cellulosic biomasses to produce valuable compounds is an ideal approach but hydrolysis of recalcitrant cellulose is complex and time-consuming. Several cellulolytic bacteria produced multienzyme complexes called “cellulosomes” that efficiently degrade the cellulose. Hence, we engineered the yeast <jats:italic>Kluyveromyces marxianus</jats:italic> to express the “largest cellulolytic complex,” which can accommodate up to 63 enzymes, on its cell surface. Due to the synergistic effects of cellulase in cellulosomes, our engineered yeast exhibited greater degradation efficiency and released significantly higher quantities of reducing sugars and ethanol from cellulosic substrates than any previous constructs. In future, this superb cellulosome complex may also be used for the synthesis of various biopharmaceutical products (e.g., astaxanthin and morphine) which involve multiple enzymatic conversion steps. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 117 (5), 2385-2394, 2020-01-17
Proceedings of the National Academy of Sciences