Single‐cell molecular profiling provides a high‐resolution map of basophil and mast cell development

DOI Web Site Web Site 4 Citations
  • Fiona K. Hamey
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Winnie W.Y. Lau
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Iwo Kucinski
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Xiaonan Wang
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Evangelia Diamanti
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Nicola K. Wilson
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Berthold Göttgens
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK
  • Joakim S. Dahlin
    Department of Haematology Wellcome–Medical Research Council Cambridge Stem Cell Institute University of Cambridge Cambridge UK

Description

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Basophils and mast cells contribute to the development of allergic reactions. Whereas these mature effector cells are extensively studied, the differentiation trajectories from hematopoietic progenitors to basophils and mast cells are largely uncharted at the single‐cell level.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We performed multicolor flow cytometry, high‐coverage single‐cell RNA sequencing analyses, and cell fate assays to chart basophil and mast cell differentiation at single‐cell resolution in mouse.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Analysis of flow cytometry data reconstructed a detailed map of basophil and mast cell differentiation, including a bifurcation of progenitors into two specific trajectories. Molecular profiling and pseudotime ordering of the single cells revealed gene expression changes during differentiation. Cell fate assays showed that multicolor flow cytometry and transcriptional profiling successfully predict the bipotent phenotype of a previously uncharacterized population of peritoneal basophil‐mast cell progenitors.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>A combination of molecular and functional profiling of bone marrow and peritoneal cells provided a detailed road map of basophil and mast cell development. An interactive web resource was created to enable the wider research community to explore the expression dynamics for any gene of interest.</jats:p></jats:sec>

Journal

  • Allergy

    Allergy 76 (6), 1731-1742, 2020-11-05

    Wiley

Citations (4)*help

See more

Report a problem

Back to top