Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers

  • Ruoyu Zhong
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Shujie Yang
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Giovanni Stefano Ugolini
    Department of Pharmaceutical Sciences Faculty, School of Pharmacy Northeastern University Palo Alto CA 94301 USA
  • Ty Naquin
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Jinxin Zhang
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Kaichun Yang
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Jianping Xia
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA
  • Tania Konry
    Department of Pharmaceutical Sciences Faculty, School of Pharmacy Northeastern University Palo Alto CA 94301 USA
  • Tony Jun Huang
    Thomas Lord Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA

抄録

<jats:title>Abstract</jats:title><jats:p>Droplet microfluidics has revolutionized the biomedical and drug development fields by allowing for independent microenvironments to conduct drug screening at the single cell level. However, current microfluidic sorting devices suffer from drawbacks such as high voltage requirements (e.g., >200 Vpp), low biocompatibility, and/or low throughput. In this article, a single‐phase focused transducer (SPFT)‐based acoustofluidic chip is introduced, which outperforms many microfluidic droplet sorting devices through high energy transmission efficiency, high accuracy, and high biocompatibility. The SPFT‐based sorter can be driven with an input power lower than 20 Vpp and maintain a postsorting cell viability of 93.5%. The SPFT sorter can achieve a throughput over 1000 events per second and a sorting purity up to 99.2%. The SPFT sorter is utilized here for the screening of doxorubicin cytotoxicity on cancer and noncancer cells, proving its drug screening capability. Overall, the SPFT droplet sorting device shows great potential for fast, precise, and biocompatible drug screening.</jats:p>

収録刊行物

  • Small

    Small 17 (46), 2021-10-17

    Wiley

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