Depth-Dependent Optical Stiffness Toward Water-Air Interface

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<jats:p>This research attempted to quantify the optical stiffness of trapped polystyrene bead toward water-air interface. The optical tweezers with 975 nm wavelength was used to optically trap a single 3 micron diameter of bead in a water-contained cell with air exposed top water surface. The optical stiffness was justified on effective radius (r*) of the bead lateral spatial distribution. The scattered light signals due to the trapped bead at different laser focus height from the bottom glass-water interface (less than 20 µm) and laser trapping powers (1.7 mW to 7.5 mW) were analyzed to investigate the r* profile. It was found that within our experimental condition, r* was independent of focus height at fixed power and exponentially decay with respect to laser power at fixed focus height.</jats:p>

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