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Estimation of Extracellular Matrix Production Using a Cultured-Chondrocyte-Based Gate Ion-Sensitive Field-Effect Transistor
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- Hiroto Satake
- Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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- Toshiya Sakata
- Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Description
Autologous chondrocytes are generally evaluated by mechanical, histological, and biochemical methods. However, the analyzed data are from discontinuous measurements at an end point, during which living chondrocytes are destroyed and stained. To solve the problem, we developed a label-free and noninvasive method of estimating extracellular matrix (ECM) production that results from chondrocyte metabolic activities using a cultured-chondrocyte-based gate ion-sensitive field-effect transistor (ISFET) in this study. Using the cultured-chondrocyte-based gate ISFET sensor, we found that the change in electrical signal (Δ
Journal
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- Analytical Chemistry
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Analytical Chemistry 91 (24), 16017-16022, 2019-11-21
American Chemical Society (ACS)
