Studying Hemichordata WBR Using Ptychodera flava

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<jats:title>Abstract</jats:title><jats:p>Hemichordates are benthic marine invertebrates closely related to chordates. Several species, including <jats:italic>Ptychodera flava</jats:italic> in the phylum Hemichordates, can undergo whole body regeneration from a small fragment. <jats:italic>P</jats:italic>. <jats:italic>flava</jats:italic> is widely distributed in the warm Indo-Pacific region and is easily collected in the lower tidal zone of a shallow beach with a coral reef. Here, we describe the methods for animal collection and preparation of regenerating tissues. The prepared tissues can be used for various molecular and/or histological experiments. We also demonstrate how to examine gene expression patterns in the tissues using whole mount in situ hybridization.</jats:p>

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