A neutralization assay with a severe fever with thrombocytopenia syndrome virus strain that makes plaques in inoculated cells
書誌事項
- 公開日
- 2017-06
- 資源種別
- journal article
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- DOI
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- 10.1016/j.jviromet.2017.01.005
- 公開者
- Elsevier BV
この論文をさがす
説明
Severe fever with thrombocytopenia syndrome (SFTS) is a recently-discovered, potentially fatal infectious disease caused by SFTS virus (SFTSV). Due to the inability of SFTSV to make clear cytopathic effects (CPE) in cell culture, titration and neutralization assays of the virus require immunostaining of inoculated cells; consequently, the assays are time-consuming and expensive. In this report, we demonstrate the use of a highly-passaged SFTSV strain, p50-2, in a neutralization assay, which made clear plaques in inoculated Vero cells under neutral red staining. Furthermore, we performed molecular analyses to determine the characteristics of the strain. The results suggested that a single amino acid mutation within the viral glycoprotein conferred the ability to make clear plaques to SFTSV.
収録刊行物
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- Journal of Virological Methods
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Journal of Virological Methods 244 4-10, 2017-06
Elsevier BV
