Rho-Signaling-Directed YAP/TAZ Activity Underlies the Long-Term Survival and Expansion of Human Embryonic Stem Cells
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説明
Human embryonic stem cells (hESCs) can survive and proliferate for an extended period of time in culture, but unlike that of tumor-derived cells, this form of cellular immortality does not depend on genomic aberrations. In this study, we sought to elucidate the molecular basis of this long-term growth property of hESCs. We found that the survival of hESCs depends on the small GTPase Rho and its activator AKAP-Lbc. We show that AKAP-Lbc/Rho signaling sustains the nuclear function of the transcriptional cofactors YAP and TAZ by modulating actin microfilament organization. By inducing reprogramming and differentiation, we found that dependency on this Rho signaling pathway is associated with the pluripotent state. Thus, our findings show that the capacity of hESCs to undergo long-term expansion in vitro is intrinsically coupled to their cellular identity through interconnected molecular circuits that link cell survival to pluripotency.
収録刊行物
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- Cell Stem Cell
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Cell Stem Cell 17 (4), 448-461, 2015-10
Elsevier BV
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キーワード
- rho GTP-Binding Proteins
- Cell Survival
- Human Embryonic Stem Cells
- A Kinase Anchor Proteins
- Nuclear Proteins
- Cell Cycle Proteins
- Cell Biology
- Actin Cytoskeleton
- Actin Depolymerizing Factors
- Genetics
- Molecular Medicine
- Humans
- Cell Self Renewal
- Acyltransferases
- Cells, Cultured
- Cell Proliferation
- Transcription Factors
詳細情報 詳細情報について
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- CRID
- 1360565166122033408
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- ISSN
- 19345909
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- PubMed
- 26321201
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
