Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen
書誌事項
- 公開日
- 2014-04-07
- 資源種別
- journal article
- 権利情報
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- http://creativecommons.org/licenses/by-nc-nd/3.0
- DOI
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- 10.1038/ncomms4591
- 公開者
- Springer Science and Business Media LLC
説明
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.
収録刊行物
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- Nature Communications
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Nature Communications 5 (1), 3591-, 2014-04-07
Springer Science and Business Media LLC
