Mutations in the deubiquitinase gene USP8 cause Cushing's disease

書誌事項

公開日
2014-12-08
資源種別
journal article
権利情報
  • http://www.springer.com/tdm
DOI
  • 10.1038/ng.3166
公開者
Springer Science and Business Media LLC

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説明

Cushing's disease is caused by corticotroph adenomas of the pituitary. To explore the molecular mechanisms of endocrine autonomy in these tumors, we performed exome sequencing of 10 corticotroph adenomas. We found somatic mutations in the USP8 deubiquitinase gene in 4 of 10 adenomas. The mutations clustered in the 14-3-3 protein binding motif and enhanced the proteolytic cleavage and catalytic activity of USP8. Cleavage of USP8 led to increased deubiqutination of the EGF receptor, impairing its downregulation and sustaining EGF signaling. USP8 mutants enhanced promoter activity of the gene encoding proopiomelanocortin. In summary, our data show that dominant mutations in USP8 cause Cushing's disease via activation of EGF receptor signaling.

収録刊行物

  • Nature Genetics

    Nature Genetics 47 (1), 31-38, 2014-12-08

    Springer Science and Business Media LLC

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