CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites
-
- Yuki Naito
- 1 Database Center for Life Science (DBCLS), 2National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan and 3Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
-
- Kimihiro Hino
- 1 Database Center for Life Science (DBCLS), 2National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan and 3Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
-
- Kumiko Ui-Tei
- 1 Database Center for Life Science (DBCLS), 2National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan and 3Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
-
- Hidemasa Bono
- 1 Database Center for Life Science (DBCLS), 2National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan and 3Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
書誌事項
- 公開日
- 2014-12-09
- 資源種別
- journal article
- 権利情報
-
- http://creativecommons.org/licenses/by/4.0/
- DOI
-
- 10.1093/bioinformatics/btu743
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼20 nt targeted sequence. The target sequence requirements are twofold. First, the 5′-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast.</jats:p> <jats:p>Availability: Freely available at http://crispr.dbcls.jp/.</jats:p> <jats:p>Contact: y-naito@dbcls.rois.ac.jp</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p>
収録刊行物
-
- Bioinformatics
-
Bioinformatics 31 (7), 1120-1123, 2014-12-09
Oxford University Press (OUP)
