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Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated with Peptide for Biosensing Application
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- Sreenadh Sasidharan Pillai
- Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan
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- Hiroshi Yukawa
- FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan
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- Daisuke Onoshima
- Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan
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- Vasudevanpillai Biju
- Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-Cho, Takamatsu, Kagawa, Japan
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- Yoshinobu Baba
- Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan
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Description
<jats:p> Quantum dots (QDs) have recently been investigated as fluorescent probes for detecting a very small number of biomolecules and live cells; however, the establishment of molecular imaging technology with on–off control of QD fluorescence remains to be established. Here we have achieved the fluorescence off state of QDs with the conjugation of black hole quencher (BHQ) molecules intermediated with peptide by using streptavidin-QDs585 and biotin-pep-BHQ-1. The fluorescence of streptavidin-QDs585 was decreased by the addition of biotin-pep-BHQ-1 in a dose-dependent manner. It has been suggested that the decrease in QDs585 fluorescence occurred through a Förster resonance energy transfer (FRET) mechanism from the analysis of fluorescence intensity and lifetime of streptavidin-QDs585 and QDs585-pep-BHQ-1. QDs585 fluorescence could be quenched by more than 60% efficiency in this system. The sequence of intermediate peptide (pep) was GPLGVRGK, which can be cleaved by matrix metalloproteinases (MMPs) produced by cancer cells. QDs585-pep-BHQ-1 is thus expected to detect the MMP production by the recovery of QDs585 fluorescence as a new bioanalytical agent for molecular imaging. </jats:p>
Journal
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- Cell Medicine
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Cell Medicine 8 (1-2), 57-62, 2015-08
SAGE Publications
