A palmtop‐sized microfluidic cell culture system driven by a miniaturized infusion pump
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- Naoki Sasaki
- Department of Chemical and Biological Sciences Faculty of Science, Japan Women's University Mejirodai, Bunkyo‐ku Tokyo Japan
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- Mika Shinjo
- Department of Chemical and Biological Sciences Faculty of Science, Japan Women's University Mejirodai, Bunkyo‐ku Tokyo Japan
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- Satoshi Hirakawa
- Department of Dermatology Hamamatsu University School of Medicine, Handayama Higashi‐ku, Hamamatsu, Shizuoka Japan
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- Masahiro Nishinaka
- Department of Applied Chemistry Graduate School of Engineering, The University of Tokyo Hongo, Bunkyo‐ku Tokyo Japan
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- Yo Tanaka
- Department of Applied Chemistry Graduate School of Engineering, The University of Tokyo Hongo, Bunkyo‐ku Tokyo Japan
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- Kazuma Mawatari
- Department of Applied Chemistry Graduate School of Engineering, The University of Tokyo Hongo, Bunkyo‐ku Tokyo Japan
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- Takehiko Kitamori
- Department of Applied Chemistry Graduate School of Engineering, The University of Tokyo Hongo, Bunkyo‐ku Tokyo Japan
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- Kae Sato
- Department of Chemical and Biological Sciences Faculty of Science, Japan Women's University Mejirodai, Bunkyo‐ku Tokyo Japan
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説明
<jats:p>A palmtop‐sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 × 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (<jats:styled-content style="fixed-case">HMEC</jats:styled-content>‐1) and human umbilical vein endothelial cells (<jats:styled-content style="fixed-case">HUVEC</jats:styled-content>) were cultured in the system. <jats:styled-content style="fixed-case">HMEC</jats:styled-content>‐1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. <jats:styled-content style="fixed-case">HUVEC</jats:styled-content> in the system oriented along the direction of the fluid flow. Claudin‐5, a tight junction protein, was localized along the peripheries of the <jats:styled-content style="fixed-case">HUVEC</jats:styled-content>. We expect that the present system is applicable to various cell types as a stand‐alone and easy‐to‐use system for microfluidic bioanalysis.</jats:p>
収録刊行物
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- ELECTROPHORESIS
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ELECTROPHORESIS 33 (12), 1729-1735, 2012-06-28
Wiley
