TectoRNP: self‐assembling RNAs with peptide recognition motifs as templates for chemical peptide ligation

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  • Kohei Yamashita
    Department of Chemistry and Biochemistry Graduate School of Engineering, Kyushu University Moto‐oka 744, Nishi‐ku Fukuoka 819‐0395 Japan
  • Takahiro Tanaka
    Department of Chemistry and Biochemistry Graduate School of Engineering, Kyushu University Moto‐oka 744, Nishi‐ku Fukuoka 819‐0395 Japan
  • Hiroyuki Furuta
    Department of Chemistry and Biochemistry Graduate School of Engineering, Kyushu University Moto‐oka 744, Nishi‐ku Fukuoka 819‐0395 Japan
  • Yoshiya Ikawa
    Department of Chemistry and Biochemistry Graduate School of Engineering, Kyushu University Moto‐oka 744, Nishi‐ku Fukuoka 819‐0395 Japan

抄録

<jats:p>TectoRNA, an artificial RNA with self‐assembling ability, has been employed as a structural platform for RNA nanotechnology and RNA synthetic biology. In this study, tectoRNA was applied as a specific template for chemical peptide ligation. On the basis of a self‐assembling tectoRNA, we designed and constructed a template RNA that facilitates peptide ligation depending on controlled dimer formation. Two RNA‐binding peptides were recognized by two peptide‐binding RNA motifs embedded in the template RNA, and chemical ligation was promoted because of the entropic effect of Mg<jats:sup>2+</jats:sup>‐dependent dimerization. In a series of biochemical analyses, we determined the relationship between the structures of the tectoRNA‐based templates and the extent of acceleration in peptide ligation. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.</jats:p>

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