Visualized macrophage dynamics and significance of S100A8 in obese fat

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  • Ryohei Sekimoto
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Shiro Fukuda
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Norikazu Maeda
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Yu Tsushima
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Keisuke Matsuda
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Takuya Mori
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Hideaki Nakatsuji
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Hitoshi Nishizawa
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Ken Kishida
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Junichi Kikuta
    Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Yumiko Maijima
    Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Tohru Funahashi
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Masaru Ishii
    Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Iichiro Shimomura
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;

抄録

<jats:title>Significance</jats:title> <jats:p> Infiltration of immune cells into adipose tissue has been observed in obesity, but, until now, these data were based on immunohistological microscopic analysis. Here, to our knowledge for the first time, we applied an intravital multiphoton imaging technique to adipose tissue with lysozyme M-EGFP transgenic (LysM <jats:sup>EGFP</jats:sup> ) mice whose EGFP was expressed in the myelomonocytic lineage. Mobility of LysM <jats:sup>EGFP</jats:sup> -positive macrophages was activated just 5 d after high-fat and high-sucrose (HF/HS) diet before the development of obesity. Furthermore, a significant increase of S100A8, one of the alarmins, was detected in fat tissue just 5 d after HF/HS diet. S100A8 stimulated chemotactic migration, and neutralization of S100A8 suppressed the HF/HS diet-induced activation of LysM <jats:sup>EGFP</jats:sup> -positive cells. Time-lapse intravital imaging first identified the very early event exhibiting increased mobility of adipose macrophages. </jats:p>

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