Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem
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- Hideyuki Doi
- Graduate School of Simulation Studies, University of Hyogo, 7-1-28, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
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- Izumi Katano
- School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike-Honcho, Himeji 670-0092, Japan
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- Yusuke Sakata
- School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike-Honcho, Himeji 670-0092, Japan
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- Rio Souma
- Graduate School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike-Honcho, Himeji 670-0092, Japan
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- Toshihiro Kosuge
- Pacific Consultants Co., Ltd, 3-22, Kanda-Nishikicho, Chiyoda-ku, Tokyo 101-8462, Japan
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- Mariko Nagano
- Graduate School of Simulation Studies, University of Hyogo, 7-1-28, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
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- Kousuke Ikeda
- Pacific Consultants Co., Ltd, 3-22, Kanda-Nishikicho, Chiyoda-ku, Tokyo 101-8462, Japan
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- Koki Yano
- Department of Biology, Faculty of Science, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621, Japan
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- Koji Tojo
- Department of Biology, Faculty of Science, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621, Japan
抄録
<jats:p> The use of environmental DNA (eDNA) has recently been employed to evaluate the distribution of various aquatic macroorganisms. Although this technique has been applied to a broad range of taxa, from vertebrates to invertebrates, its application is limited for aquatic insects such as aquatic heteropterans <jats:italic>. Nepa hoffmanni</jats:italic> (Heteroptera: Nepidae) is a small (approx. 23 mm) aquatic heteropteran that inhabits wetlands, can be difficult to capture and is endangered in Japan. The molecular tool eDNA was used to evaluate the species distribution of <jats:italic>N. hoffmanni</jats:italic> in comparison to that determined using hand-capturing methods in two regions of Japan. The eDNA of <jats:italic>N. hoffmanni</jats:italic> was detected at nearly all sites (10 eDNA-detected sites out of 14 sites), including sites where <jats:italic>N. hoffmanni</jats:italic> was not captured by hand (five eDNA-detected sites out of six captured sites). Thus, this species-specific eDNA technique can be applied to detect small, sparsely distributed heteropterans in wetland ecosystems. In conclusion, eDNA could be a valuable technique for the detection of aquatic insects inhabiting wetland habitats, and could make a significant contribution to providing distribution data necessary to species conservation. </jats:p>
収録刊行物
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- Royal Society Open Science
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Royal Society Open Science 4 (7), 170568-, 2017-07
The Royal Society