Microinjection of DNA Constructs into <i>Xenopus</i> Embryos for Gene Misexpression and <i>cis</i>-Regulatory Module Analysis

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Abstract

<jats:p>Introducing exogenous DNA into an embryo can promote misexpression of a gene of interest via transcription regulated by an attached enhancer-promoter. This protocol describes plasmid DNA microinjection into <jats:italic>Xenopus</jats:italic> embryos for misexpression of genes after zygotic gene expression begins. It also describes a method for coinjecting a reporter plasmid with mRNA or antisense morpholinos to perform luciferase reporter assays, which are useful for quantitative analysis of <jats:italic>cis</jats:italic>-regulatory sequences responding to endogenous or exogenous stimuli in embryos.</jats:p>

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