Microinjection of DNA Constructs into <i>Xenopus</i> Embryos for Gene Misexpression and <i>cis</i>-Regulatory Module Analysis

抄録

<jats:p>Introducing exogenous DNA into an embryo can promote misexpression of a gene of interest via transcription regulated by an attached enhancer-promoter. This protocol describes plasmid DNA microinjection into <jats:italic>Xenopus</jats:italic> embryos for misexpression of genes after zygotic gene expression begins. It also describes a method for coinjecting a reporter plasmid with mRNA or antisense morpholinos to perform luciferase reporter assays, which are useful for quantitative analysis of <jats:italic>cis</jats:italic>-regulatory sequences responding to endogenous or exogenous stimuli in embryos.</jats:p>

収録刊行物

参考文献 (46)*注記

もっと見る

関連プロジェクト

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ