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- Md Abdus Shabur Talukder
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
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- Ahmed Zaky Balboula
- Department of Theriogenology , Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
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- Takahiro Shirozu
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
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- Sung Woo Kim
- Animal Genetic Resources Research Center , National Institute of Animal Science, Namwon, Korea
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- Hiroki Kunii
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
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- Toshiyuki Suzuki
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
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- Tsukino Ito
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
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- Koji Kimura
- Graduate School of Environmental and Life Science , Okayama University, Okayama, Japan
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- Masashi Takahashi
- Laboratory of Animal Genetics and Reproduction , Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan
書誌事項
- 公開日
- 2018-06-01
- 資源種別
- journal article
- 権利情報
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- https://academic.oup.com/pages/standard-publication-reuse-rights
- DOI
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- 10.1530/rep-18-0078
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>In ruminants, interferon-tau (IFNT)-mediated expression of interferon-stimulated genes in peripheral blood leukocytes (PBLs) can indicate pregnancy. Recently, type 1 IFN-mediated activation of lysosomes and lysosomal cathepsins (CTSs) was observed in immune cells. This study investigated the status of lysosomal CTSs and lysosomes in PBLs collected from pregnant (P) and non-pregnant (NP) dairy cows, and conducted in vitro IFNT stimulation of NP blood leukocytes. Blood samples were collected 0, 7, 14 and 18 days post-artificial insemination, and the peripheral blood mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs) separated. The fluorescent activity of CTSB and CTSK in PMNs significantly increased with the progress of pregnancy, especially on day 18. In vitro supplementation of IFNT significantly increased the activities of CTSB and CTSK in NP PBMCs and PMNs. CTSB expression was significantly higher in PBMCs and PMNs collected from P day-18 cows than from NP cows, whereas there was no difference in CTSK expression. IFNT increased CTSB expression but did not affect CTSK expression. Immunodetection showed an increase of CTSB in P day-18 PBMCs and PMNs. In vitro stimulation of IFNT increased CTSB in NP PBMCs and PMNs. Lysosomal acidification showed a significant increase in P day-18 PBMCs and PMNs. IFNT also stimulated lysosomal acidification. Expressions of lysosome-associated membrane protein (LAMP) 1 and LAMP2 were significantly higher in P day-18 PBMCs and PMNs. The results suggest that pregnancy-specific activation of lysosomal functions by CTS activation in blood leukocytes is highly associated with IFNT during maternal and fetal recognition of pregnancy.</jats:p>
収録刊行物
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- Reproduction
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Reproduction 155 (6), 515-528, 2018-06-01
Oxford University Press (OUP)