The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching

DOI DOI PDF Web Site XML 被引用文献9件 参考文献87件
  • Brittany L Carroll
    Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, United States
  • Tatsuro Nishikino
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan
  • Wangbiao Guo
    Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, United States
  • Shiwei Zhu
    Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, United States
  • Seiji Kojima
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan
  • Michio Homma
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan
  • Jun Liu
    Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, United States

説明

<jats:p>The bacterial flagellar motor switches rotational direction between counterclockwise (CCW) and clockwise (CW) to direct the migration of the cell. The cytoplasmic ring (C-ring) of the motor, which is composed of FliG, FliM, and FliN, is known for controlling the rotational sense of the flagellum. However, the mechanism underlying rotational switching remains elusive. Here, we deployed cryo-electron tomography to visualize the C-ring in two rotational biased mutants in <jats:italic>Vibrio alginolyticus</jats:italic>. We determined the C-ring molecular architectures, providing novel insights into the mechanism of rotational switching. We report that the C-ring maintained 34-fold symmetry in both rotational senses, and the protein composition remained constant. The two structures show FliG conformational changes elicit a large conformational rearrangement of the rotor complex that coincides with rotational switching of the flagellum. FliM and FliN form a stable spiral-shaped base of the C-ring, likely stabilizing the C-ring during the conformational remodeling.</jats:p>

収録刊行物

  • eLife

    eLife 9 e61446-, 2020-09-07

    eLife Sciences Publications, Ltd

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