Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics
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- Yoichiro Sogame
- National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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- Katsuhiko Kojima
- Department of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
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- Toshikazu Takeshita
- Department of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
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- Shiho Kikuchi
- Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
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- Yuto Shimada
- Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
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- Rikiya Nakamura
- Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
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- Mikihiko Arikawa
- Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
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- Seiji Miyata
- Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, Japan
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- Eiji Kinoshita
- Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8553, Japan
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- Futoshi Suizu
- Division of Cancer Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan
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- Tatsuomi Matsuoka
- Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
説明
<jats:p>Assays of protein contained in water-soluble fraction of encysting cells Colpoda cucullus Nag-1 by two-dimensional electrophoresis (2-D PAGE) and mass spectrometry (MS) revealed that the amount of β-tubulin abruptly increased in 2.5–10 h after encystment induction. Judging from the results that total α-tubulin content did not decrease much until 12 h after encystment induction, the result indicates that disassembly of microtubules may occur soon after encystment is induced. Therefore, we tried to visualize dynamics of microtubules. Immunofluorescence microscopy using anti-α-tubulin antibody indicated that disassembly of axonemal microtubules of cilia became within 1.5 h after encystment induction, and resorbed in 3 days. Although the cytoplasmic microtubules failed to be visualized clearly, encystmentdependent globulation of cells was promoted by taxol, an inhibitor of disassembly of microtubules. It is possible that a temporary formation of cytoplasmic microtubules may be involved in cell globulation. The phosphorylation level of actin (43 kDa) became slightly elevated just after encystment induction. Lepidosomes, the sticky small globes surrounding encysting cells, were vividly stained with Acti-stain 555 phalloidin, suggesting that 43-kDa actin or its homologues may be contained in lepidosomes.</jats:p>
収録刊行物
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- Acta Protozoologica
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Acta Protozoologica 59 (3-4), 107-120, 2021-03
Uniwersytet Jagiellonski - Wydawnictwo Uniwersytetu Jagiellonskiego
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詳細情報 詳細情報について
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- CRID
- 1360572092640600576
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- ISSN
- 16890027
- 00651583
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
