Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling
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- Shuji Wakatsuki
- Department of Peripheral Nervous System Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1
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- Yoko Takahashi
- Department of Peripheral Nervous System Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1
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- Megumi Shibata
- Department of Peripheral Nervous System Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1
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- Naoki Adachi
- Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 2
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- Tadahiro Numakawa
- Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 2
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- Hiroshi Kunugi
- Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 2
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- Toshiyuki Araki
- Department of Peripheral Nervous System Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1
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説明
<jats:p>The small noncoding vault RNA (vtRNA) is a component of the vault complex, a ribonucleoprotein complex found in most eukaryotes. Emerging evidence suggests that vtRNAs may be involved in the regulation of a variety of cellular functions when unassociated with the vault complex. Here, we demonstrate a novel role for vtRNA in synaptogenesis. Using an in vitro synapse formation model, we show that murine vtRNA (mvtRNA) promotes synapse formation by modulating the MAPK signaling pathway. mvtRNA is transported to the distal region of neurites as part of the vault complex. Interestingly, mvtRNA is released from the vault complex in the neurite by a mitotic kinase Aurora-A–dependent phosphorylation of MVP, a major protein component of the vault complex. mvtRNA binds to and activates MEK1 and thereby enhances MEK1-mediated ERK activation in neurites. These results suggest the existence of a regulatory mechanism of the MAPK signaling pathway by vtRNAs as a new molecular basis for synapse formation.</jats:p>
収録刊行物
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- Journal of Cell Biology
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Journal of Cell Biology 220 (2), e201911078-, 2021-01-13
Rockefeller University Press
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キーワード
- MAP Kinase Signaling System
- Down-Regulation
- Kinesins
- Post-Synaptic Density
- Oligonucleotides, Antisense
- Models, Biological
- Cell Line
- Mice, Inbred C57BL
- Mice
- Synapses
- Neurites
- Animals
- RNA, Small Untranslated
- Amino Acid Sequence
- RNA, Small Interfering
- Extracellular Signal-Regulated MAP Kinases
- Aurora Kinase A
- Protein Binding
- Vault Ribonucleoprotein Particles
詳細情報 詳細情報について
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- CRID
- 1360572092697122944
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- ISSN
- 15408140
- 00219525
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- PubMed
- 33439240
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE