Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior

DOI Web Site 被引用文献2件 参考文献36件 オープンアクセス
  • Shino Homma-Takeda
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Hitomi Fujishiro
    Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan
  • Izumi Tanaka
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Haruko Yakumaru
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Kyoko Ayama
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Akihiro Uehara
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Masakazu Oikawa
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan
  • Seiichiro Himeno
    Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan
  • Hiroshi Ishihara
    National Institute of Radiological Sciences, National Institutes for Quanum and Radiological Science and Technology, Chiba 263-8555, Japan

説明

<jats:p>Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior.</jats:p>

収録刊行物

  • Minerals

    Minerals 11 (2), 191-, 2021-02-12

    MDPI AG

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