Trimmomatic: a flexible trimmer for Illumina sequence data
-
- Anthony M. Bolger
- 1 Department Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, 2 Institut für Biologie I, RWTH Aachen, Worringer Weg 3, 52074 Aachen and 3 Institute of Bio- and Geosciences: Plant Sciences, Forschungszentrum Jülich, Leo-Brandt-Straße, 52425 Jülich, Germany
-
- Marc Lohse
- 1 Department Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, 2 Institut für Biologie I, RWTH Aachen, Worringer Weg 3, 52074 Aachen and 3 Institute of Bio- and Geosciences: Plant Sciences, Forschungszentrum Jülich, Leo-Brandt-Straße, 52425 Jülich, Germany
-
- Bjoern Usadel
- 1 Department Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, 2 Institut für Biologie I, RWTH Aachen, Worringer Weg 3, 52074 Aachen and 3 Institute of Bio- and Geosciences: Plant Sciences, Forschungszentrum Jülich, Leo-Brandt-Straße, 52425 Jülich, Germany
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance. We have developed Trimmomatic as a more flexible and efficient preprocessing tool, which could correctly handle paired-end data.</jats:p> <jats:p>Results: The value of NGS read preprocessing is demonstrated for both reference-based and reference-free tasks. Trimmomatic is shown to produce output that is at least competitive with, and in many cases superior to, that produced by other tools, in all scenarios tested.</jats:p> <jats:p>Availability and implementation: Trimmomatic is licensed under GPL V3. It is cross-platform (Java 1.5+ required) and available at http://www.usadellab.org/cms/index.php?page=trimmomatic</jats:p> <jats:p>Contact: usadel@bio1.rwth-aachen.de</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p>
収録刊行物
-
- Bioinformatics
-
Bioinformatics 30 (15), 2114-2120, 2014-04-01
Oxford University Press (OUP)