Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine

DOI Web Site 4 Citations
  • Donna E. Akiyoshi
    <!--label omitted: 1-->BioTransplant, Incorporated, Charlestown,1 and
  • Maria Denaro
    <!--label omitted: 1-->BioTransplant, Incorporated, Charlestown,1 and
  • Haihong Zhu
    <!--label omitted: 1-->BioTransplant, Incorporated, Charlestown,1 and
  • Julia L. Greenstein
    <!--label omitted: 1-->BioTransplant, Incorporated, Charlestown,1 and
  • Papia Banerjee
    <!--label omitted: 1-->BioTransplant, Incorporated, Charlestown,1 and
  • Jay A. Fishman
    <!--label omitted: 2-->Infectious Disease and Transplant Units, Massachusetts General Hospital and Harvard Medical School, Boston,2 Massachusetts

Abstract

<jats:title>ABSTRACT</jats:title><jats:p>Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for<jats:italic>gag</jats:italic>,<jats:italic>pol</jats:italic>, and<jats:italic>env</jats:italic>in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attach- ment) region of<jats:italic>env</jats:italic>. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.</jats:p>

Journal

  • Journal of Virology

    Journal of Virology 72 (5), 4503-4507, 1998-05

    American Society for Microbiology

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