The Mouse GATA-2 Gene is Expressed in the Para-Aortic Splanchnopleura and Aorta-Gonads and Mesonephros Region

DOI DOI PDF PubMed 被引用文献7件
  • Naoko Minegishi
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Jun Ohta
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Hironori Yamagiwa
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Norio Suzuki
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Shimako Kawauchi
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Yinghui Zhou
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Satoru Takahashi
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Norio Hayashi
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • James Douglas Engel
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.
  • Masayuki Yamamoto
    From the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan; Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston IL.

説明

<jats:title>Abstract</jats:title><jats:p>We previously reported that the mouse GATA-2 gene is regulated by two alternative promoters (Minegishi et al, J Biol Chem, 273:3625, 1998). Although the more proximal IG (general) promoter is active in almost all GATA-2–expressing cells, the distal IS (specific) promoter activity was selectively detected in hematopoietic tissues but not in other mesodermal tissues. We report here in vivo analysis of the GATA-2 locus and its regulatory characteristics in hematopoietic tissues of transgenic mice. Transgenes containing 6 or 7 kbp of sequence flanking the 5′ end of the IS first exon direct expression of β-galactosidase or green fluorescent protein (GFP) reporter genes specifically to the para-aortic splanchnopleura, aorta-gonads, and mesonephros (AGM) region, and in the neural tissues. In situ hybridization analysis showed that reporter gene expression specifically recapitulates the endogenous expression profile of GATA-2 in these tissues. The flk-1, CD34, c-kit, and CD45 antigens were identified in the GFP-positive cells from the AGM region and fetal liver, indicating that GATA-2 is expressed in immature hematopoietic cells. Deletion of 3.5 kbp from the 5′ end of the 6.0 kbp IS promoter construct, including one of the DNase I hypersensitive sites, completely abolished hematopoietic expression. These experiments describe an early developmental GATA-2 hematopoietic enhancer located between 6.0 and 2.5 kbp 5′ to the IS exon.</jats:p>

収録刊行物

  • Blood

    Blood 93 (12), 4196-4207, 1999-06-15

    American Society of Hematology

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