Evaluation of Free Radical Scavenging Activities of Antioxidants with an H<sub>2</sub>O<sub>2</sub>/NaOH/DMSO System by Electron Spin Resonance
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- Yoshihiro Yoshimura
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
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- Tomoko Inomata
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
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- Hiroyuki Nakazawa
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
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- Hiroaki Kubo
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
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- Fumio Yamaguchi
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
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- Toshiaki Ariga
- Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan; Laboratory of Analytical Chemistry, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0071, Japan; and Research and Development Division of Kikkoman Corporation, Noda 399, Noda-shi, Chiba Prefecture 278-0037, Japan
書誌事項
- 公開日
- 1999-10-06
- DOI
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- 10.1021/jf990422w
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
An H(2)O(2)/NaOH/DMSO system has been developed for the formation of three free radicals, and the application of the system was examined with the antioxidants ascorbic acid and tocopherol. Superoxide anion, hydroxyl radical,and methyl radical are simultaneously generated in this system. The scavenging activity of ascorbic acid and tocopherol for these radicals was estimated by 5, 5'-dimethyl-1-pyrroline-N-oxide spin trapping electron spin resonance. Both water-soluble and oil-soluble antioxidants could be evaluated by using this system. Ascorbic acid specifically inhibited the superoxide anion and hydroxyl radical, whereas tocopherol suppressed the methyl radical.
収録刊行物
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- Journal of Agricultural and Food Chemistry
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Journal of Agricultural and Food Chemistry 47 (11), 4653-4656, 1999-10-06
American Chemical Society (ACS)