DNA Methylation in Embryo Development: Epigenetic Impact of ART (Assisted Reproductive Technologies)
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- Sebastian Canovas
- Physiology of Reproduction Group University of Murcia Murcia Spain
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- Pablo J. Ross
- Department of Animal Science UC Davis Davis CA USA
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- Gavin Kelsey
- Epigenetics Programme The Babraham Institute Cambridge UK
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- Pilar Coy
- Physiology of Reproduction Group University of Murcia Murcia Spain
説明
<jats:sec><jats:label /><jats:p>DNA methylation can be considered a component of epigenetic memory with a critical role during embryo development, and which undergoes dramatic reprogramming after fertilization. Though it has been a focus of research for many years, the reprogramming mechanism is still not fully understood. Recent results suggest that absence of maintenance at DNA replication is a major factor, and that there is an unexpected role for TET3‐mediated oxidation of 5mC to 5hmC in guarding against de novo methylation. Base‐resolution and genome‐wide profiling methods are enabling more comprehensive assessments of the extent to which ART might impair DNA methylation reprogramming, and which sequence elements are most vulnerable. Indeed, as we also review here, studies showing the effect of culture media, ovarian stimulation or embryo transfer on the methylation pattern of embryos emphasize the need to face ART‐associated defects and search for strategies to mitigate adverse effects on the health of ART‐derived children.</jats:p></jats:sec>
収録刊行物
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- BioEssays
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BioEssays 39 (11), 2017-09-21
Wiley