Identification and characterization of mutations in housefly (Musca domestica) acetylcholinesterase involved in insecticide resistance

  • Sinéad B. WALSH
    Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, Herts. AL5 2JQ, U.K.
  • Tracey A. DOLDEN
    Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, Herts. AL5 2JQ, U.K.
  • Graham D. MOORES
    Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, Herts. AL5 2JQ, U.K.
  • Michael KRISTENSEN
    Danish Pest Infestation Laboratory, Skovbrynet 14, DK-2800 Lyngby, Denmark
  • Terence LEWIS
    Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berks. RG42 6ET, U.K.
  • Alan L. DEVONSHIRE
    Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, Herts. AL5 2JQ, U.K.
  • Martin S. WILLIAMSON
    Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, Herts. AL5 2JQ, U.K.

抄録

<jats:p>Acetylcholinesterase (AChE) insensitive to organophosphate and carbamate insecticides has been identified as a major resistance mechanism in numerous arthropod species. However, the associated genetic changes have been reported in the AChE genes from only three insect species; their role in conferring insecticide insensitivity has been confirmed, using functional expression, only for those in Drosophila melanogaster. The housefly, Musca domestica, was one of the first insects shown to have this mechanism; here we report the occurrence of five mutations (Val-180 → Leu, Gly-262 → Ala, Gly-262 → Val, Phe-327 → Tyr and Gly-365 → Ala) in the AChE gene of this species that, either singly or in combination, confer different spectra of insecticide resistance. The baculovirus expression of wild-type and mutated housefly AChE proteins has confirmed that the mutations each confer relatively modest levels of insecticide insensitivity except the novel Gly-262 → Val mutation, which results in much stronger resistance (up to 100-fold) to certain compounds. In all cases the effects of mutation combinations are additive. The mutations introduce amino acid substitutions that are larger than the corresponding wild-type residues and are located within the active site of the enzyme, close to the catalytic triad. The likely influence of these substitutions on the accessibility of the different types of inhibitor and the orientation of key catalytic residues are discussed in the light of the three-dimensional structures of the AChE protein from Torpedo californica and D. melanogaster.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ