Estimation of Creatinine by the Jaffe Reaction

  • Harry Husdan
    Metabolic-Renal Unit and the Department of Biochemistry, Toronto Western Hospital, and the Departments of Medicine and of Pathological Chemistry, University of Toronto, Toronto, Canada
  • Abraham Rapoport
    Metabolic-Renal Unit and the Department of Biochemistry, Toronto Western Hospital, and the Departments of Medicine and of Pathological Chemistry, University of Toronto, Toronto, Canada

Description

<jats:title>Abstract</jats:title> <jats:p>Total chromogen, true, and AutoAnalyzer methods of measuring serum and urine creatinine by the Jaffe reaction were investigated. Some factors influencing this reaction were examined. These included wavelength, blank, linearity, and conditions of color development. Modifications of the three methods were made and their precision, recovery, and sample stability determined. The interference of ketones and glucose were measured. Finally, the values obtained by the three methods on the same samples of serum and urine were compared statistically.</jats:p>

Journal

  • Clinical Chemistry

    Clinical Chemistry 14 (3), 222-238, 1968-03-01

    Oxford University Press (OUP)

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