Whole-genome resequencing shows numerous genes with nonsynonymous SNPs in the Japanese native cattle Kuchinoshima-Ushi
Abstract
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Because the Japanese native cattle <jats:italic>Kuchinoshima-Ushi</jats:italic> have been isolated in a small island and their lineage has been intensely protected, it has been assumed to date that numerous and valuable genomic variations are conserved in this cattle breed.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>In this study, we evaluated genetic features of this breed, including single nucleotide polymorphism (SNP) information, by whole-genome sequencing using a Genome Analyzer II. A total of 64.2 Gb of sequence was generated, of which 86% of the obtained reads were successfully mapped to the reference sequence (Btau 4.0) with BWA. On an average, 93% of the genome was covered by the reads and the number of mapped reads corresponded to 15.8-fold coverage across the covered region. From these data, we identified 6.3 million SNPs, of which more than 5.5 million (87%) were found to be new. Out of the SNPs annotated in the bovine sequence assembly, 20,432 were found in protein-coding regions containing 11,713 nonsynonymous SNPs in 4,643 genes. Furthermore, phylogenetic analysis using sequence data from 10 genes (more than 10 kbp) showed that <jats:italic>Kuchinoshima-Ushi</jats:italic> is clearly distinct from European domestic breeds of cattle.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>These results provide a framework for further genetic studies in the <jats:italic>Kuchinoshima-Ushi</jats:italic> population and research on functions of SNP-containing genes, which would aid in understanding the molecular basis underlying phenotypic variation of economically important traits in cattle and in improving intrinsic defects in domestic cattle breeds.</jats:p> </jats:sec>
Journal
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- BMC Genomics
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BMC Genomics 12 (1), 2011-02-10
Springer Science and Business Media LLC
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Details 詳細情報について
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- CRID
- 1360574094669862400
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- ISSN
- 14712164
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- Data Source
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- Crossref